Technologies
PBCF houses the instruments listed below. Instrument time is reserved using the Idea Elan X platform.
For access or training on PBCF instrumentation and Idea Elan X, contact the PBCF Director, Jerry Chang.
New users are welcomed.
Biophysical Instrumentation
High-throughput plate reader and imager with neighboring incubator.
Multi-zone, temperature-controlled for up to 4 samples and 4 references.
Circular dichroism for structural and thermodynamic properties.
Steady state fluorescence and quantum yield measurements.
Fluorimeter for steady-state, lifetime, and phosphorescence experiments.
High-throughput, temperature-controlled SLS and DLS plate reader.
Label-free, real-time analysis of biomolecular interactions.
Rapidly acquire spectral (190-850 nm) data with microvolume quantities.
Real time visualization of single-molecule interactions.
Rapid mass determination for 30 kDa to 5 MDa molecules.
Phosphor imaging, RGB fluorescence, near-IR fluorescence, and OD.
Lattice SIM and SMLS for 2D and 3D live structures.
Offline workstation to process data using instrumentation software.
Biochemistry Instrumentation
High-pressure homogenizer to rapidly reduce particles from micron to nanometer sizes.
Gradient maker and piston fractionator allows for separation and collection of solutions.
High-speed centrifuge with fixed-angle rotors with capacity for 50 and 500 mL containers.
Ultracentrifuge operating at up to 100K RPM speeds. Swing bucket, fixed-angle, & zonal rotors available.
Temperature-controlled shaker accommodating up to 6 liters, speeds up to 500 RPM.
-80°C lyophilizer to remove water and acetonitrile only, no organics/acids. Containers provided when available.
Temperature-adjustable (-10°C to 40°C) centrifuge. Swing bucket and fixed-angle rotors available.
Impact pulverize samples at -80°C, capacity to pulverize multiple samples simultaneously.
Preparative-HPLC system used for reverse-phase separation and fractionation.