Instrument

The PBCF houses the instruments listed below.  Instrument time is reserved using the PBCF Idea Elan. For access to the PBCF and the Idea Elan system, or to coordinate training, contact the manager Jerry Chang.  New users are welcome.

Instruments

BioTek Neo2 Plate Reader 

  • Multimode plate reader good for UV-Vis absorbance, fluorescence intensity, luminescence, fluorescence polarization, time-resolved fluorescence, and alpha detection

  • Protocol

Chirascan V100 Spectrometer

  • High sensitivity and accuracy Circular Dichroism (CD) Spectrometer

  • For any use of the CD instrument that results in a publication, please acknowledge NIH award 1S10OD025102-01

  • Protocol 

Horiba Lifetime fluorimeter

  • Light source

    • Light source75W Xenon arc lamp

    • Xenon flash lamp

    • 420 PP-FD pulsed white laser

    • White laser doubler

  • Detector

    • 920C PMT

    • InGaAs detector

    • InAs detector

    • PPD 850

  • Steady-state wavelength

    • 200-900 nm

    • 1000 nm - 3400 nm

  • Lifetime wavelength

    • 260-360 nm

    • 400-900 nm

  • Phosphorescence 

    • 1000-1500 nm

MicroCal Auto iTC 200 

  • Automated, high sensitive isothermal titration calorimeter to characterize molecular interactions without labeling

  • Sample Preparation Guideline

Dynamic Light Scattering (DLS)

  • Wyatt DynaPro NanoStar DLS system

  • Stand-alone mode and online mode with Wyatt SEC-MALS

Zeiss Elyra 7 Super-resolution microscope 

Combine Lattice Structured Illumination Microscopy (SIM) with Single Molecule Localization Microscopy (SMLM)

  • Applications:

    • Live cells: resolve structural details in 2D or 3D and multiple colors

    • Live organisms: resolve structural details in 2D or 3D with high penetration depth over large areas

    • Fixed specimens:  investigate the arrangement of cellular components and proteins, explore the interaction of molecules

  • Components:

    • Objectives: 63x/1.46 TIRF, 63x/1.40 Oil, 63x/1.20 W, and 40x/1.2 W

    • Laser: 405 nm, 488 nm, 561, nm and 640 nm

    • Incubator: dark laser safe heated, humidity-controlled, CO2 Chamber

Amersham Typhoon 5 Biomolecular Imager

  • A versatile, accurate quantitation, and high-resolution imaging system, supporting phosphor imaging, RGB fluorescence, near-infrared fluorescence, and Densitometry (OD). 

  • Excitation: 488 nm, 532 nm, 635 nm, 685 nm, and 785 nm

  • Emission filter: 390BP,Cy2 525BP20, Cy3 570BP20, Cy5 670BP30, IRshort 720BP20, and IRlong 825PB30

  • Application: Fluorescence detection and quantification, Western blots, Two-dimensional difference gel electrophoresis (2D-DIGE), radioactivity detection, and densitometry

  • Protocol 

Autoclaves

BioComp Gradient Forming and Gradient Fractionation System

  • Gradient Master :  generates linear gradients using patented process, tilted tube rotation.  By layering two end point solutions in the tube, the mater takes several minutes to make a gradient. Solutes include  sucrose, glycerol, tartarate, Optiprep®, Nycodenz®, Ficoll®, Percoll®, Metrizamide, Renografin®, NaCl, CsCl, NaAc, and KCl.

  • Piston Fractionation: leave the gradient undisturbed while piston descends from the top, displacing layers and keeping the flow smooth and precise. High resolution gradient analysis.

  • Application: Ribosome purification and profiling

  • Available for SW-41 rotors

  • Tubes: SETON 7030 (14 X 89 mm)

  • Protocol 

Cell Disruptors 

  • Emulsiflex C3 high-pressure homogenizer 

    • High-Pressure Homogenizer Continuous flow capacity: 3L/h 

    • Pressure: up to 30,000 psi 

    • Minimum volume: 10 mL 

    • Applications: cell disruption, emulsion & liposome processing

    • Protocol 

  • SPEX SamplePrep 6875D Freezer/Mill® Dual Chamber Cryogenic Grinder ​​​​​​

 

 

 

Incubator Shakers

  • Innova 43 Shaker

High-Speed Centrifuges

  • Beckman Avanti J-E Centrifuge

  • Sorvall Desktop Centrifuge

Lyophilizer 

Ultracentrifuges  

  • Beckman Optima 100 Ultracentrifuge 

    • Swing bucket rotors: SW 60, SW 32, SW41

    • Fixed angle rotors: Type 100, Type 42.2, Type 70.1Type 70

    • Zonal rotor: Ti-15  

    • Protocol